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Journal / Bioengineering / 2D og 3D matricer til at studere lineær…
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Bioengineering
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JoVE Journal Bioengineering
2D og 3D matricer til at studere lineær invadosomdannelse og aktivitet
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2D og 3D matricer til at studere lineær invadosomdannelse og aktivitet

Article DOI: 10.3791/54911-v • 12:26 min • June 2nd, 2017
June 2nd, 2017 •
*1,2, *1, 1,3,4, 2, 2, 1, 1
1INSERM U1053, 2Icahn School of Medicine at Mount Sinai, Tisch Cancer Institute, 3Faculté de Médecine, Casablanca, Université Mohammed 6 des Sciences de la Santé (UM6SS), 4Laboratoire National de Référence
* These authors contributed equally

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Denne protokol beskriver, hvordan man forbereder en 2D-blandet matrix, der består af gelatine og kollagen I, og et 3D-collagen-I-stik for at studere lineære invadosomer. Disse protokoller tillader undersøgelsen af ​​lineær invadosomdannelse, matrixnedbrydningsaktivitet og invasionskapaciteterne af primære celler og cancercellelinier.

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Bioengineering udgave 124 Lineær invadosom ekstracellulær matrix nedbrydningsaktivitet collagen I 3D invasion
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