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Mærkning af Breast Cancer Patient-afledte Xenografter med Sporbare Journalister for tumorvækst og Metastase Studies

Article DOI: 10.3791/54944-v • 09:53 min • November 30th, 2016
November 30th, 2016 •
1, 1, 1, 1, 1
1Department of Pathology, School of Medicine, University of Colorado Anschutz Medical Campus

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Vi beskriver en fremgangsmåde til stabil mærkning af patient-afledte xenotransplantater (PDXs) med lentivirale partikler, der udtrykker grønt-fluorescerende protein og luciferase reportere. Denne fremgangsmåde muliggør sporing af væksten af PDXs ved det primære sted, samt detektering spontane og eksperimentelle metastaser anvendelse af in vivo-billeddannelse.

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Cancer Research PDX Patient-afledt-xenografter lentiviral luciferase transplantation musemodeller metastaser
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