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Induceren van ischemie-reperfusie schade in de Mouse Ear Skin voor intravitale Multiphoton Imaging van immuunresponsen

Article DOI: 10.3791/54956-v • 06:29 min • December 22nd, 2016
December 22nd, 2016 •
1,2, 1, 3, 4,5,6, 2,7, 1,2,5,8
1Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Biopolis, 2Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, 3Lee Kong Chian School of Medicine, Nanyang Technological University, 4Centenary Institute for Cancer Medicine and Cell Biology, 5Discipline of Dermatology, University of Sydney, 6Department of Dermatology, Royal Prince Alfred Hospital, 7LSI Immunology Programme, National University of Singapore, 8School of Biological Sciences, Nanyang Technological University

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Dit protocol beschrijft de inductie van een ischemie-reperfusie (IR) model op de muis oor huid met behulp van magneet klemmen. Met behulp van een custom-built intravitale imaging model, bestuderen we in vivo ontstekingsreacties post-reperfusie. De reden voor de ontwikkeling van deze techniek is om te begrijpen hoe leukocyten reageren op huid IR schade verlengen.

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Geneeskunde huid immunologie ischemie-reperfusie ontsteking interstitiële migratie multiphoton microscopie intravitale imaging
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