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JoVE Journal Genetics
Engenharia de fatores artificiais para manipular Especificamente splicing alternativo em células humanas
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Engenharia de fatores artificiais para manipular Especificamente splicing alternativo em células humanas

Article DOI: 10.3791/54967-v • 10:06 min • April 26th, 2017
April 26th, 2017 •
*1, *1, 2, 1, 1, 1, 1
1Key Laboratory of Computational Biology, CAS-MPG Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences (SIBS), 2Institute of Cancer Stem Cell, Second Affiliated Hospital, Cancer Center, Dalian Medical University
* These authors contributed equally

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Este relatório descreve um método de bioengenharia para conceber e construir novos factores de splicing artificial (ASFS) que modulam especificamente a splicing de genes alvo em células de mamífero. Este método pode ser expandido para manipular vários fatores artificiais para manipular outros aspectos do metabolismo do ARN.

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Genética Edição 122 fatores de ligação ARN artificiais engenharia protéica fatores de splicing splicing alternativo andaime PUF proteínas de ligação a ARN câncer
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