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JoVE Journal Genetics
Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus
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Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

Article DOI: 10.3791/54976-v • 09:57 min • December 28th, 2016
December 28th, 2016 •
1,2, 1, 1,2
1Entomology Department, University of Maryland, 2Program in Molecular and Cell Biology, University of Maryland

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Summary
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Here, we present protocols for rearing an intermediate-germ beetle, Dermestes maculatus (D. maculatus) in the lab. We also share protocols for embryonic and parental RNAi and methods for analyzing embryonic phenotypes to study gene function in this species.

Tags

Gene Function Dermestes Maculatus RNA Interference Developmental Biology Evolutionary Biology Insect Model System Rearing Cage Wood Shavings Styrofoam Larvae Pupate Adult Beetles Wet Cat Food Embryo Collection Incubator
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