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JoVE Journal
Neuroscience

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生活多巴胺能神经元可靠的鉴定中脑培养使用RNA测序和TH-启动子驱动的EGFP表达
 
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生活多巴胺能神经元可靠的鉴定中脑培养使用RNA测序和TH-启动子驱动的EGFP表达

Article DOI: 10.3791/54981-v 10:54 min February 10th, 2017
February 10th, 2017

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Summary

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帕金森病(PD),黑质(SNC)多巴胺能神经元退化,导致运动功能障碍。此处我们报告从表达eGFP的小鼠通过酪氨酸羟化酶(TH)的启动子序列驱动的培养腹侧中脑神经元,从培养收获个别荧光神经元,并使用RNA-SEQ测量它们转录的协议。

Tags

神经科学,第120,单细胞,RNA测序,细胞收集,中脑培养物,多巴胺能神经元,帕金森氏病
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