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Høj opløsning respirometri at vurdere mitokondrie funktion i permeabiliseret og intakte celler

Article DOI: 10.3791/54985-v • 08:33 min • February 8th, 2017
February 8th, 2017 •
1, 1
1Department of Intensive Care Medicine, Inselspital, Bern University Hospital, University of Bern

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Høj opløsning respirometri anvendes til at bestemme mitokondrie iltforbrug. Dette er en enkel teknik til at bestemme mitochondriske respiratorisk kæde-komplekser '(I-IV) respirationsfrekvenser, maksimal mitokondriel elektrontransport systemkapaciteten, og mitokondriel ydre membran integritet.

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Cellular Biology mitokondrier oxidativ fosforylering iltforbrug høj opløsning respirometri cytochrom C digitonin
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