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The Use of Ex Vivo Whole-organ Imaging and Quantitative Tissue Histology to Determine the Bio-distribution of Fluorescently Labeled Molecules
JoVE Journal
Biology
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JoVE Journal Biology
The Use of Ex Vivo Whole-organ Imaging and Quantitative Tissue Histology to Determine the Bio-distribution of Fluorescently Labeled Molecules
DOI:

08:07 min

December 24, 2016

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Chapters

  • 00:05Title
  • 00:46Whole Organ Ex Vivo Fluorescence Imaging
  • 02:00Quantitative Tissue and Standard Histology Preparation
  • 04:22Tissue and Standard Sectioning
  • 05:47Results: Representative Ex Vivo Whole Organ Fluorescence and Quantitative Tissue Histology Analyses
  • 07:15Conclusion

Summary

Automatic Translation

Ex vivo whole-organ imaging is a rapid method for determining the relative concentrations of fluorescently labeled compounds within and between tissues or treatment groups. Conversely, quantitative fluorescence histology, while more labor intensive, allows for the quantification of the absolute tissue levels of labeled molecules.

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