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Journal / Neuroscience / Low-Density Primäre Hippocampus Kultur
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Neuroscience
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Low-Density Primäre Hippocampus Kultur

Article DOI: 10.3791/55000-v • 17:47 min • April 18th, 2017
April 18th, 2017 •
*1,2, *1,2, 1,2
1Department of Physiology and Pathophysiology, University of Manitoba, 2Kleysen Institute for Advanced Medicine, Health Sciences Centre
* These authors contributed equally

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Dieser Artikel beschreibt das Protokoll zur Kultivierung niedriger Dichte primären hippocampalen Neuronen wachsen auf Glasdeckgläschen über einen einschichtigen glial invertiert. Das Neuron und Glia-Schichten werden durch Paraffinwachsperlen abgetrennt. Die Neuronen durch diese Verfahren gezüchtet sind geeignet für hochauflösende optische Bildgebung und funktionelle Assays.

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Neuroscience Ausgabe 122 primäre Neuronen Astroglia einschichtige Sandwich-Kultur Banker Methode Hippocampus Low-Density-Kultur zelluläre Neurobiologie
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