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JoVE Journal Cancer Research
Teste Vaskulær Invasive Evne av kreftceller i Sebrafisk (
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Teste Vaskulær Invasive Evne av kreftceller i Sebrafisk (

Article DOI: 10.3791/55007-v • 07:31 min • November 3rd, 2016
November 3rd, 2016 •
1, 1, 1, 1
1Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University

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Denne metoden benytter sebrafisk embryoer for effektivt å teste den vaskulære invasiv evne til kreftceller. Fluorescent kreftceller blir injisert i precardiac sinus eller plommesekken utviklings embryoer. Kreftcelle vaskulær invasjon og ekstravasering blir vurdert via fluorescens mikroskopi av halen området 24 til 96 timer senere.

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Cancer Research kreft Ekstravasasjon Sebrafisk Embryo fluorescens Vaskulær Invasion Metastase
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