Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Na diferenciação in vitro de células T CD4 +…
JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content.

JoVE Journal Immunology and Infection
Na diferenciação in vitro de células T CD4 + humano FOXP3 + Induzida T reguladoras (iTregs) a partir de células naive T CD4 + usando um protocolo de TGF-β contendo
Establishment and Evaluation of a Mouse Catheter-Related Infection Model
Journal (Immunology and Infection)
Establishment and Evaluation of a Mouse Catheter-Related Infection Model
A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells
Journal (Immunology and Infection)
A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells
 
Click here for the English version

Na diferenciação in vitro de células T CD4 + humano FOXP3 + Induzida T reguladoras (iTregs) a partir de células naive T CD4 + usando um protocolo de TGF-β contendo

Article DOI: 10.3791/55015-v • 08:20 min • December 30th, 2016
December 30th, 2016 •
1, *1, *1, 1
1Unit of Computational Medicine, Center for Molecular Medicine, Department of Medicine Solna, Karolinska Institutet, Karolinska University Hospital, & Science for Life Laboratory
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Este protocolo descreve a geração reprodutível e fenotipagem de células T reguladoras humanas induzida (iTregs) a partir de células naive T CD4 + in vitro. Diferentes protocolos de indução de FOXP3 permitem o estudo de fenótipos específicos iTreg obtidos com os respectivos protocolos.

Tags

Immunology 118 Edição células T reguladoras Treg células T CD4 + o isolamento de células magnético, citocinas FOXP3 TGF-β IL-2 citometria de fluxo intracelular qRT-PCR
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter