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Immunology and Infection

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JoVE Journal Immunology and Infection
Flow Virometry to Analyze Antigenic Spectra of Virions and Extracellular Vesicles
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Flow Virometry to Analyze Antigenic Spectra of Virions and Extracellular Vesicles

Article DOI: 10.3791/55020-v • 06:28 min • January 25th, 2017
January 25th, 2017 •
1, 1, 1, 2, 3, 1
1Section on Intercellular Interactions, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, 2Laboratory of Atherothrombosis, Cardiology Department, Moscow State University of Medicine and Dentistry, 3Sidra Medical and Research Center

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Summary
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Viruses or extracellular vesicles were immunocaptured with 15 nm magnetic nanoparticles coupled to antibodies recognizing surface antigens. The captured virions or vesicles were labeled with fluorescent antibodies against other surface antigens. The resultant complexes were separated in high magnetic field and analyzed with conventional flow cytometers triggered on fluorescence.

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Flow Virometry Antigenic Spectra Virions Extracellular Vesicles Magnetic Particles MNPs Flow Cytometer Phenotype Function Human Diseases Viral Infection In-bulk Analysis Single Virions Single Extracellular Vesicles Insight Test Of Viral Particles Antibodies Proper Set-up Microcentrifuge Tube Incubate Continuous Mixing Prewet Concentrator
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