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Journal / Developmental Biology / Indsamling af serum- og Feeder-fri mus embryonale …
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JoVE Journal Developmental Biology
Indsamling af serum- og Feeder-fri mus embryonale stamceller-conditioned Medium for en celle-fri tilgang
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Indsamling af serum- og Feeder-fri mus embryonale stamceller-conditioned Medium for en celle-fri tilgang

Article DOI: 10.3791/55035-v • 09:32 min • January 8th, 2017
January 8th, 2017 •
1, 2,3, 1
1Department of Biochemistry and Molecular Biology and Smart-aging Convergence Research Center, College of Medicine, Yeungnam University, 2Physiology and Experimental Medicine Program, The Hospital for Sick Children Research Institute, 3Department of Laboratory Medicine and Pathobiology, University of Toronto

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Denne protokol giver en metode til indsamling af musen embryonale stamceller (Mesc) -konditioneret medium (Mesc-CM) afledt af serum (føtalt bovint serum, FBS) - og feeder (mus embryonale fibroblaster, MEF'er) -fri betingelser for en celle -fri tilgang. Det kan være anvendelig til behandling af aldring og aldring-associerede sygdomme.

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Developmental Biology Cellular degeneration i Konditionerede medier (CM) Embryonale stamceller (EKSF) Cell-free tilgang Serumfrit fødefri
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