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Developmental Biology

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Indsamling af serum- og Feeder-fri mus embryonale stamceller-conditioned Medium for en celle-fri tilgang
 

Indsamling af serum- og Feeder-fri mus embryonale stamceller-conditioned Medium for en celle-fri tilgang

Article doi: 10.3791/55035
January 8th, 2017 Usage Statistics

Summary January 8th, 2017

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Denne protokol giver en metode til indsamling af musen embryonale stamceller (Mesc) -konditioneret medium (Mesc-CM) afledt af serum (føtalt bovint serum, FBS) - og feeder (mus embryonale fibroblaster, MEF'er) -fri betingelser for en celle -fri tilgang. Det kan være anvendelig til behandling af aldring og aldring-associerede sygdomme.

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