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JoVE Journal Developmental Biology
Recogida de suero y libre de alimentador madre embrionarias de ratón medio celular acondicionado para un enfoque libre de células
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Recogida de suero y libre de alimentador madre embrionarias de ratón medio celular acondicionado para un enfoque libre de células

Article DOI: 10.3791/55035-v • 09:32 min • January 8th, 2017
January 8th, 2017 •
1, 2,3, 1
1Department of Biochemistry and Molecular Biology and Smart-aging Convergence Research Center, College of Medicine, Yeungnam University, 2Physiology and Experimental Medicine Program, The Hospital for Sick Children Research Institute, 3Department of Laboratory Medicine and Pathobiology, University of Toronto

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Este protocolo proporciona un método para la obtención de células madre embrionarias de ratón (MESC) -conditioned medio (mESC-CM) derivado de suero (suero bovino fetal, FBS) - y alimentadoras (fibroblastos de embriones de ratón, MEFs) -free condiciones para una célula enfoque exento. Puede ser aplicable para el tratamiento de envejecimiento y las enfermedades de envejecimiento asociado.

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Biología del Desarrollo No. 119 La senescencia celular medio condicionado (CM) las células madre embrionarias (CME) el enfoque libre de células libre de suero libre de alimentador
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