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JoVE Journal Chemistry
In Situ Detectie en eencellige kwantificering van metaaloxide nanodeeltjes met behulp van nucleaire Microprobe analyse
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In Situ Detectie en eencellige kwantificering van metaaloxide nanodeeltjes met behulp van nucleaire Microprobe analyse

Article DOI: 10.3791/55041-v • 14:54 min • February 3rd, 2018
February 3rd, 2018 •
*1,2, *1,2, 1,2, 1,2, 1,2, 1,2, 3,4, 1,2
1Centre d'Etudes Nucléaires Bordeaux Gradignan (CENBG), Université de Bordeaux, 2Centre d'Etudes Nucléaires Bordeaux Gradignan (CENBG), CNRS, 3Institut de Chimie de la Matière Condens é e de Bordeaux (ICMCB), CNRS, 4Institut de Chimie de la Matière Condens é e de Bordeaux (ICMCB), Université de Bordeaux
* These authors contributed equally

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Beschrijven we een procedure voor de opsporing van chemische elementen aanwezig in situ in menselijke cellen, alsmede hun kwantificering in vitro . De methode is geschikt voor elk celtype en is vooral nuttig voor de kwantitatieve chemische analyses in afzonderlijke cellen na in vitro metaaloxide nanodeeltjes blootstelling.

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Chemie kwestie 132 nanodeeltjes scheikundig Element Mapping In Situ kwantificering nucleaire Microprobe analyse ImageJ IBA-J eencellige analyse fluorescentie microscopie
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