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Immunology and Infection

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JoVE Journal Immunology and Infection
Dekstran forbedrer Lentiviral signaltransduksjon effektiviteten av Murine og menneskelig primære NK celler
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Dekstran forbedrer Lentiviral signaltransduksjon effektiviteten av Murine og menneskelig primære NK celler

Article DOI: 10.3791/55063-v • 08:42 min • January 15th, 2018
January 15th, 2018 •
1, 2, 3, 4, 5, 6, 6, 1,5,6,7
1Laboratory of Molecular Immunology and Immunotherapy, Blood Research Institute, The Blood Center of Wisconsin, 2Vector Core Lab, Blood Research Institute, The Blood Center of Wisconsin, 3Laboratory of Lymphocyte Biology, Blood Research Institute, The Blood Center of Wisconsin, 4Laboratory of Stem Cell Transcriptional Regulation, Blood Research Institute, The Blood Center of Wisconsin, 5Department of Microbiology and Immunology, The Medical College of Wisconsin, 6Department of Pediatrics, The Medical College of Wisconsin, 7Department of Medicine, The Medical College of Wisconsin

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Målet med denne studien var å formulere teknologi som tillater for vellykket genet signaltransduksjon i primære naturlig killer () Celler NK. Den dekstran-mediert lentiviral signaltransduksjon menneske eller musen primære NK celler resulterer i høyere gene expression effektivitet. Denne metoden av genet signaltransduksjon vil vesentlig forbedre NK cellen genetisk manipulasjon.

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Immunologi problemet 131 signaltransduksjon primære NK celler dekstran lentivirus genmodifiserte immunterapi
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