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Journal / Immunology and Infection / Dextran effektiviserer Lentiviral transduktion af …
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Immunology and Infection

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JoVE Journal Immunology and Infection
Dextran effektiviserer Lentiviral transduktion af Murine og menneskelige primære NK-celler
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Dextran effektiviserer Lentiviral transduktion af Murine og menneskelige primære NK-celler

Article DOI: 10.3791/55063-v • 08:42 min • January 15th, 2018
January 15th, 2018 •
1, 2, 3, 4, 5, 6, 6, 1,5,6,7
1Laboratory of Molecular Immunology and Immunotherapy, Blood Research Institute, The Blood Center of Wisconsin, 2Vector Core Lab, Blood Research Institute, The Blood Center of Wisconsin, 3Laboratory of Lymphocyte Biology, Blood Research Institute, The Blood Center of Wisconsin, 4Laboratory of Stem Cell Transcriptional Regulation, Blood Research Institute, The Blood Center of Wisconsin, 5Department of Microbiology and Immunology, The Medical College of Wisconsin, 6Department of Pediatrics, The Medical College of Wisconsin, 7Department of Medicine, The Medical College of Wisconsin

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Målet med denne undersøgelse var at formulere teknologier, der muliggør vellykket gen transduktion i primære natural killer (NK) celler. Dextran-medieret lentiviral transduktion af menneskelige eller musen primære NK celler resultater i højere gen expression effektivitet. Denne metode af genet transduktion vil langt forbedre NK celler genetisk manipulation.

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Immunologi sag 131 transduktion primære NK celler dextran lentivirus genetisk modificeret immunterapi
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