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Journal / Immunology and Infection / Beeldvorming van de neutrofielen phagosome en…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Beeldvorming van de neutrofielen phagosome en cytoplasma Met behulp van een Ratiometrische pH Indicator
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Beeldvorming van de neutrofielen phagosome en cytoplasma Met behulp van een Ratiometrische pH Indicator

Article DOI: 10.3791/55107-v • 12:42 min • April 5th, 2017
April 5th, 2017 •
1, 1, 1, 2, 1
1Centre for Molecular Medicine, Division of Medicine, University College London, 2Cell and Developmental Biology, University College London

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Dit manuscript beschrijft een eenvoudige methode om de pH en phagosomal gebied en het cytoplasmatische pH van mens en muis neutrofielen via de ratiometrische indicator seminaphthorhodafluor (SNARF) -1 of S-1 te meten. Dit wordt bereikt met behulp van levende cellen confocale fluorescentie microscopie en beeldanalyse.

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Immunologie fagocytose neutrofielen phagosomal pH ratiometrische beeldvorming fluorescentiemicroscopie aangeboren immuniteit
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