Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Imaging den Neutrofil fagosomet og Cytoplasma Ved …
JoVE Journal
Immunology and Infection

This content is Open Access.

JoVE Journal Immunology and Infection
Imaging den Neutrofil fagosomet og Cytoplasma Ved hjælp af en Ratiometrisk pH-indikator
Fecal (micro) RNA Isolation
Journal (Immunology and Infection)
Fecal (micro) RNA Isolation
Mouse- and Human-derived Primary Gastric Epithelial Monolayer Culture for the Study of Regeneration
Journal (Immunology and Infection)
Mouse- and Human-derived Primary Gastric Epithelial Monolayer Culture for the Study of Regeneration
 
Click here for the English version

Imaging den Neutrofil fagosomet og Cytoplasma Ved hjælp af en Ratiometrisk pH-indikator

Article DOI: 10.3791/55107-v • 12:42 min • April 5th, 2017
April 5th, 2017 •
1, 1, 1, 2, 1
1Centre for Molecular Medicine, Division of Medicine, University College London, 2Cell and Developmental Biology, University College London

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Dette manuskript beskriver en enkel metode til at måle fagosomale pH og område samt den cytoplasmatiske pH af humane og muse neutrofiler ved hjælp af den ratiometriske indikator seminaphthorhodafluor (snarf) -1, eller S-1. Dette opnås ved hjælp af live-cell konfokal fluorescens mikroskopi og billedanalyse.

Tags

Immunologi fagocytose neutrofil fagosomale pH ratiometrisk billeddannelse fluorescensmikroskopi medfødt immunitet
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter