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Journal / Immunology and Infection / Imaging o neutrófilos fagossoma e citoplasma…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Imaging o neutrófilos fagossoma e citoplasma utilizando um indicador de pH Ratiometric
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Imaging o neutrófilos fagossoma e citoplasma utilizando um indicador de pH Ratiometric

Article DOI: 10.3791/55107-v • 12:42 min • April 5th, 2017
April 5th, 2017 •
1, 1, 1, 2, 1
1Centre for Molecular Medicine, Division of Medicine, University College London, 2Cell and Developmental Biology, University College London

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Este manuscrito descreve um método simples para medir o pH e phagosomal área, bem como o pH citoplasmática de neutrófilos humanos e de rato utilizando o indicador de medida proporcional seminaphthorhodafluor (SNARF) -1, ou S-1. Isto é conseguido usando microscopia de fluorescência confocal ao vivo de células e análise de imagem.

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Immunology edição 122 fagocitose neutrófilos pH phagosomal imagiologia raciométrica microscopia de fluorescência a imunidade inata
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