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Biology
Biology
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开源高内涵分析利用自动荧光寿命成像显微镜
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Article
DOI:
10.3791/55119-v
•
09:30 min
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January 18th, 2017
开源高内涵分析利用自动荧光寿命成像显微镜
January 18th, 2017
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Frederik Görlitz*1, Douglas J. Kelly*1, Sean C. Warren1, Dominic Alibhai2, Lucien West3, Sunil Kumar1, Yuriy Alexandrov1, Ian Munro1, Edwin Garcia1, James McGinty1, Clifford Talbot1, Remigiusz A. Serwa4, Emmanuelle Thinon4, Vincenzo da Paola3, Edward J. Murray5, Frank Stuhmeier6, Mark A. A. Neil1, Edward W. Tate4, Christopher Dunsby1,7, Paul M. W. French1
1Photonics Group, Department of Physics, Imperial College London, 2Institute for Chemical Biology, Department of Chemistry, Imperial College London, 3MRC Clinical Sciences Centre, Hammersmith Hospital, 4Chemical Biology Section, Department of Chemistry, Imperial College London, 5Retroscreen Virology Ltd, 6Pfizer Global Research and Development, Pfizer Limited, Sandwich, Kent, UK, 7Centre for Histopathology, Imperial College London
* These authors contributed equally
Chapters
Summary
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我们目前使用的测定使用荧光共振能量转移(FRET)的读数蛋白质相互作用自动荧光寿命成像(FLIM)一个开源的高内涵分析(HCA)的仪器。此openFLIM-HCA仪数据采集是通过软件写入μManager和数据分析在FLIMfit进行控制。