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Biology

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JoVE Journal Biology
Open Source High Content Analysis Door gebruik te maken van de automaten Fluorescence Lifetime Imaging Microscopy
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Open Source High Content Analysis Door gebruik te maken van de automaten Fluorescence Lifetime Imaging Microscopy

Article DOI: 10.3791/55119-v • 09:30 min • January 18th, 2017
January 18th, 2017 •
*1, *1, 1, 2, 3, 1, 1, 1, 1, 1, 1, 4, 4, 3, 5, 6, 1, 4, 1,7, 1
1Photonics Group, Department of Physics, Imperial College London, 2Institute for Chemical Biology, Department of Chemistry, Imperial College London, 3MRC Clinical Sciences Centre, Hammersmith Hospital, 4Chemical Biology Section, Department of Chemistry, Imperial College London, 5Retroscreen Virology Ltd, 6Pfizer Global Research and Development, Pfizer Limited, Sandwich, Kent, UK, 7Centre for Histopathology, Imperial College London
* These authors contributed equally

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We presenteren een open source hoog gehalte analyse (HCA) instrument gebruik te maken van geautomatiseerde fluorescentie lifetime imaging (FLIM) voor het testen van eiwit interacties met behulp van Förster resonance energy transfer (FRET) op basis van uitlezingen. Data-acquisitie voor deze openFLIM-HCA instrument wordt bestuurd door software geschreven in μManager en data-analyse wordt uitgevoerd in FLIMfit.

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Biofysica fluorescentie microscopie FLIM HCA open source FRET geautomatiseerde microscopie drug discovery
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