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Open Source High Content Analysis hjælp Automated Fluorescens Lifetime Imaging Microscopy

Article DOI: 10.3791/55119-v • 09:30 min • January 18th, 2017
January 18th, 2017 •
*1, *1, 1, 2, 3, 1, 1, 1, 1, 1, 1, 4, 4, 3, 5, 6, 1, 4, 1,7, 1
1Photonics Group, Department of Physics, Imperial College London, 2Institute for Chemical Biology, Department of Chemistry, Imperial College London, 3MRC Clinical Sciences Centre, Hammersmith Hospital, 4Chemical Biology Section, Department of Chemistry, Imperial College London, 5Retroscreen Virology Ltd, 6Pfizer Global Research and Development, Pfizer Limited, Sandwich, Kent, UK, 7Centre for Histopathology, Imperial College London
* These authors contributed equally

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Vi præsenterer et open source højt indhold analyse (HCA) instrument udnytter automatiseret fluorescens levetid imaging (FLIM) til analyse protein interaktioner ved hjælp Förster resonans (FRET) baserede udlæsninger. Erhvervelse Data til denne openFLIM-HCA instrument styres af software skrevet i μManager og foretages analyse af data i FLIMfit.

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Biofysik fluorescens mikroskopi Flim HCA open source FRET automatiseret mikroskopi lægemiddelforskning
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