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Open Source alto contenuto di analisi Utilizzando Automated Fluorescence Lifetime Imaging Microscopy
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Open Source alto contenuto di analisi Utilizzando Automated Fluorescence Lifetime Imaging Microscopy

Article DOI: 10.3791/55119-v • 09:30 min • January 18th, 2017
January 18th, 2017 •
*1, *1, 1, 2, 3, 1, 1, 1, 1, 1, 1, 4, 4, 3, 5, 6, 1, 4, 1,7, 1
1Photonics Group, Department of Physics, Imperial College London, 2Institute for Chemical Biology, Department of Chemistry, Imperial College London, 3MRC Clinical Sciences Centre, Hammersmith Hospital, 4Chemical Biology Section, Department of Chemistry, Imperial College London, 5Retroscreen Virology Ltd, 6Pfizer Global Research and Development, Pfizer Limited, Sandwich, Kent, UK, 7Centre for Histopathology, Imperial College London
* These authors contributed equally

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Vi presentiamo un alto contenuto strumento open source di analisi (HCA) che utilizza l'imaging di fluorescenza vita automatizzato (FLIM) per saggiare le interazioni delle proteine ​​che utilizzano Förster trasferimento di energia di risonanza (FRET) letture basate. L'acquisizione dei dati per questo strumento openFLIM-HCA è controllato da un software scritto in μManager e l'analisi dei dati viene effettuata in FLIMfit.

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Biofisica microscopia a fluorescenza FLIM HCA open source tasto microscopia automatizzata scoperta di nuovi farmaci
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