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Biology

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Open Source Høy innholdsanalyse Utnytte Automated Fluorescence Lifetime Imaging Mikros
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Open Source Høy innholdsanalyse Utnytte Automated Fluorescence Lifetime Imaging Mikros

Article DOI: 10.3791/55119-v • 09:30 min • January 18th, 2017
January 18th, 2017 •
*1, *1, 1, 2, 3, 1, 1, 1, 1, 1, 1, 4, 4, 3, 5, 6, 1, 4, 1,7, 1
1Photonics Group, Department of Physics, Imperial College London, 2Institute for Chemical Biology, Department of Chemistry, Imperial College London, 3MRC Clinical Sciences Centre, Hammersmith Hospital, 4Chemical Biology Section, Department of Chemistry, Imperial College London, 5Retroscreen Virology Ltd, 6Pfizer Global Research and Development, Pfizer Limited, Sandwich, Kent, UK, 7Centre for Histopathology, Imperial College London
* These authors contributed equally

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Vi presenterer en åpen kildekode høy innholdsanalyse (HCA) instrument utnytte automatiserte fluorescens levetid imaging (FLIM) for analyse av protein interaksjoner bruker Förster resonans energi overføring (FRET) basert avlesninger. Datainnsamlingen for denne openFLIM-HCA instrument styres av programvare skrevet i μManager og dataanalyse er gjennomført i FLIMfit.

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Biofysikk fluorescens mikroskopi FLIM HCA åpen kildekode gnage automatisert mikroskopi medisiner
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