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JoVE Journal Developmental Biology
Organ Kultur og Whole Mount Immunofluorescensanalyse Farging av Muse Wolffian Kanaler
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Organ Kultur og Whole Mount Immunofluorescensanalyse Farging av Muse Wolffian Kanaler

Article DOI: 10.3791/55134-v • 09:16 min • January 13th, 2017
January 13th, 2017 •
1, 1
1Gynaecology Oncology Group, School of Biomedical Sciences and Pharmacy, University of Newcastle

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Vi presenterer en metode for isolering og kultur av Wolffian mus kanalen (WD). Vi viser også en detaljert prosedyre for hele mount farging av dyrkede / nylig isolerte WDS med fluorescently merkede antistoffer. Sammen utgjør disse teknikkene gjør det mulig å studere WD utvikling, kveiling og differensiering.

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Developmental Biology bitestikkel orgel kultur farging Wolffian duct hele mount,
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