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Journal / Immunology and Infection / Legionella pneumophila exterior vesículas de…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Legionella pneumophila exterior vesículas de membrana
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Legionella pneumophila exterior vesículas de membrana: Aislamiento y análisis de su potencial pro-inflamatoria en los macrófagos

Article DOI: 10.3791/55146-v • 08:35 min • February 22nd, 2017
February 22nd, 2017 •
1, 1, 1, 1, 1, 1,2,3
1Institute for Lung Research, Universities of Giessen and Marburg Lung Center, Philipps-University Marburg, 2German Center for Lung Research, 3Department of Medicine, Pulmonary and Critical Care Medicine, University Medical Center Giessen and Marburg

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A continuación, se describe la purificación de Legionella pneumophila (L. pneumophila) vesículas de membrana externa (OMV) a partir de cultivos líquidos. Estas vesículas purificadas se usan después para el tratamiento de los macrófagos para analizar su potencial pro-inflamatoria.

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Infección No. 120, Vesículas de membrana externa macrófagos purificación ultracentrifugación diferencial la respuesta pro-inflamatoria la replicación bacteriana
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