Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Efficace isolamento di isole funzionali da…
JoVE Journal
Developmental Biology

A subscription to JoVE is required to view this content.

JoVE Journal Developmental Biology
Efficace isolamento di isole funzionali da neonatali mouse Pancreas
An Optimized O9-1/Hydrogel System for Studying Mechanical Signals in Neural Crest Cells
Journal (Developmental Biology)
An Optimized O9-1/Hydrogel System for Studying Mechanical Signals in Neural Crest Cells
Murine Neural Plate Targeting by In Utero Nano-Injection (NEPTUNE) at Embryonic Day 7.5
Journal (Developmental Biology)
Murine Neural Plate Targeting by In Utero Nano-Injection (NEPTUNE) at Embryonic Day 7.5
 
Click here for the English version

Efficace isolamento di isole funzionali da neonatali mouse Pancreas

Article DOI: 10.3791/55160-v • 07:04 min • January 6th, 2017
January 6th, 2017 •
1, 1
1Department of Cell and Development Biology, Vanderbilt University Medical School

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Descriviamo qui un protocollo per isolare isolotti intatti da topi neonati. Pancreas sono stati parzialmente digerito con collagenasi, seguita da lavaggio e raccolta manuale. 20 - 80 clusters insule possono essere ottenuti per pancreas da topi appena nati, che sono adatti per diversi studi insulari.

Tags

Biologia dello Sviluppo Numero 119 il trapianto collagenasi il diabete la maturazione isolotti l'insulina le cellule beta la secrezione neonatale raccolta a mano
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter