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Journal / Developmental Biology / Isolamento eficaz de ilhotas funcionais a partir…
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Developmental Biology

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JoVE Journal Developmental Biology
Isolamento eficaz de ilhotas funcionais a partir de ratinho neonato Pâncreas
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Isolamento eficaz de ilhotas funcionais a partir de ratinho neonato Pâncreas

Article DOI: 10.3791/55160-v • 07:04 min • January 6th, 2017
January 6th, 2017 •
1, 1
1Department of Cell and Development Biology, Vanderbilt University Medical School

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Nós descrevemos um protocolo aqui para isolar ilhotas intactas de ratos recém-nascidos. Pâncreas foi parcialmente digerido com colagenase, seguido por lavagem e colheita manual. 20 - 80 agrupamentos de ilhéus podem ser obtidos por pâncreas de ratinhos recém-nascidos, que são adequados para vários estudos da ilhota.

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Biologia do Desenvolvimento Edição 119 transplante colagenase diabetes maturação Ilhotas insulina células beta secreção neonatal colheita de mão
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