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Samtidig Måling av mitokondrie Kalsium og Mitokondriell membranpotensialet i levende celler med fluorescerende mikros

Article DOI: 10.3791/55166-v • 08:43 min • January 24th, 2017
January 24th, 2017 •
1,2, 1, 3
1Centre for Genetic Diseases, Hudson Institute of Medical Research, 2The Department of Molecular and Translational Sciences, Monash University, 3Department of Physiology, University College London

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Mitokondrier kan utnytte det elektrokjemiske potensial over sin indre membran (Δ Ψm) binder kalsium (Ca2 +), slik at de kan forme cytosolisk Ca2 + signalering i cellen. Vi beskriver en metode for samtidig måling mitokondrier Ca 2+ opptak og ΔΨ M i levende celler ved hjelp av fluorescerende fargestoffer og konfokal mikroskopi.

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Cellular Biology Mitokondrier membranpotensiale kalsium fluorescerende flekker levende celler konfokalmikroskopi
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