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Levende celler og 1a 3D Analyse af angiotensin receptor type handel i transfekterede humane embryoniske nyreceller Brug konfokalmikroskopi
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Levende celler og 1a 3D Analyse af angiotensin receptor type handel i transfekterede humane embryoniske nyreceller Brug konfokalmikroskopi

Article DOI: 10.3791/55177-v • 09:51 min • March 27th, 2017
March 27th, 2017 •
1,2, 3, 3, 1,2, 4
1Department of Biochemistry, Georgetown University Medical Center, 2Department of Medicine, Georgetown University Medical Center, 3Department of Physics, Georgetown University Medical Center, 4Department of Oncology, Georgetown University Medical Center

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Her præsenterer vi en protokol til billedet celler, der udtrykker grønt fluorescerende protein-mærket angiotensin typen 1a-receptorer under endocytose igangsat af angiotensin II-behandling. Denne teknik omfatter mærkning lysosomer med et andet fluorescerende markør, og derefter anvender software til at analysere co-lokalisering af receptor og lysosomer i tre dimensioner over tid.

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Molecular Biology live-cell imaging laserscanning konfokal mikroskopi angiotensin type 1 receptor transfektion endosomer intracellulære vesikler tredimensionelt billedanalyse lysosomer transmembrane receptorer forbedret grønt fluorescerende protein
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