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Journal / Genetics / 약물 저항 유전자를 확인하기위한 QTL 매핑 및 CRISPR / Cas9…
JoVE Journal
Genetics

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JoVE Journal Genetics
약물 저항 유전자를 확인하기위한 QTL 매핑 및 CRISPR / Cas9 편집
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약물 저항 유전자를 확인하기위한 QTL 매핑 및 CRISPR / Cas9 편집

Article DOI: 10.3791/55185-v • 11:37 min • June 22nd, 2017
June 22nd, 2017 •
1, 2, 2
1State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, 2Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University

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Toxoplasma gondii 에서 약물 내성 유전자를 확인하는 데 유전지도를 기반으로하는 QTL 매핑을 사용하는 방법과 게놈 타겟을 효율적으로 편집하는 CRISPR / Cas9 시스템으로 어떻게 검증 할 수 있는지에 대한 세부 정보가 제공됩니다. 약물 내성 유전자.

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(QTL)지도 작성 CRISPR 가이드 RNA (gRNA) sinefungin 단일 염기 다형성 (SNP) 유전 적 교차 전체 게놈 시퀀싱 (WGS)
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