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从健康志愿者和它们的迁徙可能通过血清中的影响心脏手术后的内皮祖细胞的分离

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JoVE Journal Medicine

Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery

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从健康志愿者和它们的迁徙可能通过血清中的影响心脏手术后的内皮祖细胞的分离

DOI:

10.3791/55192-v

•

08:43 min

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February 14, 2017

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Christoph Emontzpohl², David Simons, Sandra Kraemer, Andreas Goetzenich, Gernot Marx, Jürgen Bernhagen⁶, Christian Stoppe

¹Department of Intensive Care Medicine,University Hospital Aachen, ²Institute of Biochemistry and Molecular Biology,University Hospital Aachen, ³Department of Radiology,German Cancer Research Center, ⁴Department of Thoracic and Cardiovascular Surgery,University Hospital Aachen, ⁵Department of Vascular Biology, Institute for Stroke and Dementia Research (ISD),Klinikum der Universität München, ⁶Deutsches Zentrum für Herz-/Kreislaufkrankheiten (DZHK),Munich Heart Alliance

Chapters

00:05Title
01:11Isolation of Endothelial Progenitor Cells (EPCs)
03:52Migration Assay
06:50Results: Characterization of Endothelial Progenitor Cells (EPCs)
08:20Conclusion

Summary

Automatic Translation

内皮祖细胞（EPCs）的关键参与缺血组织的新血管形成。这个方法描述了从外周血内皮祖细胞的分离，以及它们对心脏手术患者的血清样品洄游潜在的识别。

Tags

Endothelial Progenitor Cells Cardiac Surgery Cell Isolation Migration CD-34 Positive Cells Density Gradient Peripheral Blood Mononuclear Cells Magnetic Beads Cell Culture Angiogenesis Ischemia Re-perfusion Injury

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