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Journal / Developmental Biology / Regisserad differentiering av primitiv och…
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JoVE Journal Developmental Biology
Regisserad differentiering av primitiv och slutgiltig hematopoetiska progenitorceller från mänskliga pluripotenta stamceller
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Regisserad differentiering av primitiv och slutgiltig hematopoetiska progenitorceller från mänskliga pluripotenta stamceller

Article DOI: 10.3791/55196-v • 14:37 min • November 1st, 2017
November 1st, 2017 •
1, 1
1Department of Internal Medicine, Hematology Division, Washington University in St. Louis

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Här presenterar vi mänskliga pluripotenta stamceller (hPSC) kultur-protokoll, används för att differentiera hPSCs in CD34+ hematopoetiska progenitorceller. Denna metod använder scenen-specifika manipulation av kanoniska WNT signalering ange celler uteslutande att antingen slutgiltig eller primitiva hematopoetiska program.

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Utvecklingsbiologi frågan 129 pluripotenta stamceller mänskliga embryonala stamceller cellodling embryoid organ WNT signalering slutgiltiga blodbildning primitiva blodbildning hemogenic endotelet
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