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JoVE Journal
Neuroscience

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视网膜神经节细胞定义子集的单细胞RNA-Seq
 
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视网膜神经节细胞定义子集的单细胞RNA-Seq

Article DOI: 10.3791/55229-v 11:26 min May 22nd, 2017
May 22nd, 2017

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Summary

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在这里,我们提出了一种组合方法,用于在分离之前对神经元细胞类型进行分类,以及随后对单细胞转录组进行表征。该协议优化了用于成功的RNA测序(RNA-Seq)的样品的制备,并描述了专门为增强细胞多样性的理解而设计的方法。

Tags

神经科学,第123期,本质感光性视网膜神经节细胞,电生理学,RNA测序,单细胞分离,低输入RNA测序,神经元
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