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Neuroscience

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JoVE Journal Neuroscience
Single-cell RNA-Seq af definerede subsæt af retinale Ganglion-celler
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Single-cell RNA-Seq af definerede subsæt af retinale Ganglion-celler

Article DOI: 10.3791/55229-v • 11:26 min • May 22nd, 2017
May 22nd, 2017 •
1, 2, 2, 1, 2
1Department of Genetics, Development, and Cell Biology, Iowa State University, 2Department of Neurobiology, Northwestern University

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Her præsenterer vi en kombinatorisk tilgang til klassificering af neuronelle celletyper forud for isolation og til den efterfølgende karakterisering af enkeltcelletranscriptomer. Denne protokol optimerer forberedelsen af ​​prøver til vellykket RNA-sekventering (RNA-Seq) og beskriver en metodologi, der er designet specielt til forbedret forståelse af cellemangfoldighed.

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Neurovidenskab udgave 123 Intrinsically lysfølsomme retinal ganglionceller elektrofysiologi RNA-sekventering enkeltcelleisolering RNA-sekvensering med lav indgang neuroner
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