Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / Rapid Deletion Produksjon i Fungi via
JoVE Journal
Genetics

A subscription to JoVE is required to view this content.

JoVE Journal Genetics
Rapid Deletion Produksjon i Fungi via
High-Throughput Robotically Assisted Isolation of Temperature-sensitive Lethal Mutants in Chlamydomonas reinhardtii
Journal (Genetics)
High-Throughput Robotically Assisted Isolation of Temperature-sensitive Lethal Mutants in Chlamydomonas reinhardtii
Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast
Journal (Genetics)
Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast
 
Click here for the English version

Rapid Deletion Produksjon i Fungi via

Article DOI: 10.3791/55239-v • 11:24 min • June 12th, 2017
June 12th, 2017 •
1, 2, 3, 1
1Toxicology and Mycotoxin Research Unit, NPRC, USDA-ARS, 2Hazera Seeds LTD, Brurim, 3Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora” - Universidad de Málaga - Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Estación Experimental “La Mayora”

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Gene-deletjonsmutanter generert ved homolog rekombination er gullstandarden for genfunksjonsstudier. OSCAR (One Step Construction av Agrobacterium-Recombination-ready-plasmids) metode for rask generering av deletjonskonstruksjoner er beskrevet. Agrobacterium- mediert sopptransformasjon følger. Endelig presenteres en PCR-basert bekreftelsesmetode for genutslettelser i sopptransformanter.

Tags

Genetikk Utgave 124 Fungal transformasjon Fungal deletion mutant generasjon ATMT One Step Construction of
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter