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JoVE Journal
Developmental Biology

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马卵母细胞染色体分离,组蛋白乙酰化和主轴形态分析
 
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马卵母细胞染色体分离,组蛋白乙酰化和主轴形态分析

Article DOI: 10.3791/55242-v 12:11 min May 11th, 2017
May 11th, 2017

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Summary

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Click here for the English version.

该手稿描述了一种用于形态和生物化学表征马卵母细胞的实验方法。具体来说,本文通过超声引导的卵子吸收(OPU)如何收集不成熟和成熟的马卵母细胞,以及如何调查染色体分离,纺锤形态,全局组蛋白乙酰化和mRNA表达。

Tags

发育生物学,第123期,非整倍体,卵母细胞成熟,中期,减数分裂,表观遗传学,组蛋白乙酰转移酶,组蛋白脱乙酰酶,辅助繁殖,卵子吸收
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