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Developmental Biology

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JoVE Journal Developmental Biology
Analyse af kromosomsegregation, histonacetylering og spindelmorfologi i hesteococytter
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Analyse af kromosomsegregation, histonacetylering og spindelmorfologi i hesteococytter

Article DOI: 10.3791/55242-v • 12:11 min • May 11th, 2017
May 11th, 2017 •
1, 1,2, 3, 3, 4, 5, 3, 6, 1, 1, 3
1Department of Health, Animal Science and Food Safety, University of Milan, 2IRCCS. Istituto Ortopedico Galeazzi, 3PRC, CNRS, IFCE, Université de Tours, INRA, 4PAO, INRA, 5Clinique des Animaux de Compagnie et des Équidés, Université de Liège, 6University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania

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Dette manuskript beskriver en eksperimentel tilgang til morfologisk og biokemisk karakterisering af hesteocyter. Specifikt illustrerer det foreliggende arbejde, hvordan man opsamler umodne og modne hesteocyter ved hjælp af ultralydstyret ægoptagelse (OPU) og hvordan man undersøger kromosomsegregation, spindelmorfologi, global histonacetylering og mRNA-ekspression.

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Udviklingsbiologi udgave 123 aneuploidi oocytmodning metafase meiosis epigenetisk histonacetyltransferase histon-deacetylase assisteret reproduktion ægoptagelse
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