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Developmental Biology

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JoVE Journal Developmental Biology
Cell Lineage Analyser og genfunktion undersøgelser under anvendelse af Twin-spot MARCM
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Cell Lineage Analyser og genfunktion undersøgelser under anvendelse af Twin-spot MARCM

Article DOI: 10.3791/55278-v • 06:30 min • March 2nd, 2017
March 2nd, 2017 •
1,2, 1, 1, 1,2
1Institute of Cellular and Organismic Biology, Academia Sinica, 2Graduate Institute of Life Sciences, National Defense Medical Center

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Her præsenteres en protokol for en mosaik mærkningsteknik, der tillader visualisering af neuroner afledt fra en fælles progenitorcelle i to forskellige farver. Dette letter neurale afstamning analyse med mulighed for medfødte-dating individuelle neuroner og studere genfunktion i de samme neuroner i forskellige individer.

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Developmental Biology twin-spot MARCM celle afstamning analyse fødsel-ordre encellede opløsning neuronal morfologi genfunktioner
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