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Journal / Biochemistry / Preparation of Giant Vesicles Encapsulating…
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Biochemistry

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JoVE Journal Biochemistry
Preparation of Giant Vesicles Encapsulating Microspheres by Centrifugation of a Water-in-oil Emulsion
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Preparation of Giant Vesicles Encapsulating Microspheres by Centrifugation of a Water-in-oil Emulsion

Article DOI: 10.3791/55282-v • 05:43 min • January 24th, 2017
January 24th, 2017 •
1, 2, 2, 1, 2, 2,3,4
1Department of Mathematical and Physical Sciences, Faculty of Science, Japan Women's University, 2Department of Bioorganization Research, Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, 3Department of Life and Coordination-Complex Molecular Science, Institute for Molecular Science, National Institutes of Natural Sciences, 4Research Center for Complex Systems Biology, The University of Tokyo

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Summary
Automatic Translation

Giant vesicles containing highly packed micrometer-sized components are useful cell models. The water-in-oil emulsion centrifugation method is a simple, powerful tool for the preparation of giant vesicles with encapsulated materials.

Tags

Water-in-oil Emulsion Centrifugation Giant Vesicles Microspheres DOPC Texas Red DHPE Chloroform Lipid Film Liquid Paraffin Aluminum Foil Incubation Nonfluorescent Microspheres Fluorescent Microspheres Sucrose Tris Buffered Solution Deionized Water Vortexing Sonication
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