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JoVE Journal Developmental Biology
Identifikation af erythromyeloidprogenitorer og deres afkom i musembryoen ved flowcytometri
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Identifikation af erythromyeloidprogenitorer og deres afkom i musembryoen ved flowcytometri

Article DOI: 10.3791/55305-v • 08:59 min • July 17th, 2017
July 17th, 2017 •
1,2, 1, 1, 1
1Department of Developmental and Stem Cell Biology, CNRS UMR3738, Department of Immunology, Institut Pasteur, 2Cellule Pasteur UPMC, University Pierre et Marie Curie

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Selv om infiltrerende makrofager rekrutteres kontinuerligt til voksne væv fra cirkulerende forstadier, frøer residente makrofager deres væv under udvikling, hvor de opretholdes uden yderligere indløb fra forfædre. Forfædrene for residente makrofager blev for nylig identificeret. Her præsenterer vi metoder til den genetiske skæbneplanlægning af de residente makrofagprogenitorer.

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Udviklingsbiologi udgave 125 makrofager æggeblomme sac definitiv hæmatopoiesis hæmatopoietisk stamme og stamceller museembryoner FACS skæbneplanlægning
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