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A Lipid Extraction and Analysis Method for Characterizing Soil Microbes in Experiments with Many Samples

Article DOI: 10.3791/55310-v • 17:40 min • July 16th, 2017
July 16th, 2017 •
1, 2, 3, 1, 4, 1
1Department of Agronomy and Great Lakes Bioenergy Research Center, University of Wisconsin - Madison, 2Department of Soil Science, University of Wisconsin - Madison, 3Department of Soil, Water, and Climate, University of Minnesota, 4Faculty of Science and Engineering, Curtin University

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The article describes a method that increases throughput while balancing effort and accuracy for extraction of lipids from the cell membranes of microorganisms for use in characterizing both total lipids and the relative abundance of indicator lipids to determine soil microbial community structure in studies with many samples.

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Lipid Extraction Analysis Method Soil Microbes Experiments Many Samples Cell Membranes Microorganisms Total Lipids Indicator Lipids Soil Microbial Community Structure Soil Heterogeneity Soil Samples Lab Freeze Drying Storage Grinding Homogenize Labware Cleanliness GC Chromatogram
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