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Journal / Genetics / Generering av fluorescerande protein Fusioner i
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Genetics

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JoVE Journal Genetics
Generering av fluorescerande protein Fusioner i
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Generering av fluorescerande protein Fusioner i

Article DOI: 10.3791/55333-v • 09:27 min • March 4th, 2017
March 4th, 2017 •
1, 2, 1
1Department of Pediatrics, University of Minnesota, 2Department of Molecular Microbiology and Biotechnology, Tel Aviv University

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PCR-medierad gen modifiering kan användas för att generera fluorescerande proteinfusioner i Candida species, vilket underlättar visualisering och kvantifiering av jästceller och proteiner. Häri presenterar vi en strategi för att konstruera ett fluorescerande protein fusion (Eno1-FP) i Candida parapsilosis.

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Genetik , PCR-medierad genmodifiering fluorescerande protein, Litiumacetat transformation,
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