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Journal / Developmental Biology / Cultuur van volwassen transgene zebravis Retina…
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Developmental Biology

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JoVE Journal Developmental Biology
Cultuur van volwassen transgene zebravis Retina Explantaten voor Live-cell imaging van Multiphoton Microscopy
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Cultuur van volwassen transgene zebravis Retina Explantaten voor Live-cell imaging van Multiphoton Microscopy

Article DOI: 10.3791/55335-v • 11:55 min • February 24th, 2017
February 24th, 2017 •
1, 1, 1,2, 1
1Department of Biological Sciences, University of Notre Dame, 2Department of Neurosurgery, Mayo Clinic

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Zebravis netvlies regeneratie is meestal onderzocht met behulp van vaste netvliezen. Echter, dynamische processen zoals interkinetic nucleaire migratie optreden tijdens de regeneratieve respons en vereisen voor live-cell imaging om de onderliggende mechanismen te onderzoeken. We beschrijven hier de cultuur en beeldvorming voorwaarden te controleren Interkinetic Nuclear Migratie (INM) in real-time met behulp van multiphoton microscopie.

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Developmental Biology retinale explantatie multiphoton microscopie live-cell imaging zebravis interkinetic nucleaire migratie cultuur regeneratie Müller glia mitose snelheid
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