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JoVE Journal
Developmental Biology

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Culture of Adult transgene zebrafisk Retinal Eksplantater til Live-celle Imaging af multifoton Microscopy
 
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Culture of Adult transgene zebrafisk Retinal Eksplantater til Live-celle Imaging af multifoton Microscopy

Article DOI: 10.3791/55335
February 24th, 2017

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Summary February 24th, 2017

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Zebrafisk retinal regenerering er for det meste blevet undersøgt ved hjælp af faste nethinder. Imidlertid dynamiske processer såsom interkinetic nuklear migration forekomme under den regenerative respons og kræver live-cell imaging at undersøge de underliggende mekanismer. Her beskriver vi kultur og billedbehandling betingelser for at overvåge Interkinetic Nuclear Migration (INM) i realtid ved hjælp multifoton mikroskopi.

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