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Journal / Biochemistry / Multimer-PAGE: A Fremgangsmåde til registrering…
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Biochemistry

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JoVE Journal Biochemistry
Multimer-PAGE
Isolation of Tissue Extracellular Vesicles from the Liver
Journal (Biochemistry)
Isolation of Tissue Extracellular Vesicles from the Liver
Removal and Replacement of Endogenous Ligands from Lipid-Bound Proteins and Allergens
Journal (Biochemistry)
Removal and Replacement of Endogenous Ligands from Lipid-Bound Proteins and Allergens
 
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Multimer-PAGE: A Fremgangsmåde til registrering og Løsning proteinkomplekser i biologiske prøver

Article DOI: 10.3791/55341-v • 07:40 min • May 5th, 2017
May 5th, 2017 •
1, 2, 3, 3
1Physiology, Michigan State University, 2Center for Neurodegenerative Science Van Andel Institute, 3Pharmaceutical Sciences, Wayne State University

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En fremgangsmåde til stabilisering og adskillelse native proteinkomplekser fra umodificeret væv lysat anvendelse af en amin-reaktivt protein tværbindingsmiddel koblet til en hidtil ukendt todimensional polyacrylamidgelelektroforese (PAGE) system er vist.

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Biokemi Gelelektroforese proteinkomplekser separation tværbinding multimerer PAGE
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