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JoVE Journal Biochemistry
Beredning av Nukleosomer med differentiellt isotopmärkt Sister Histoner
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Beredning av Nukleosomer med differentiellt isotopmärkt Sister Histoner

Article DOI: 10.3791/55349-v • 09:26 min • March 26th, 2017
March 26th, 2017 •
1
1Department of Structural Biology, Leibniz Institute of Molecular Pharmacology (FMP-Berlin)

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Detta protokoll beskriver beredning av nukleosomer innehållande differentiellt isotopmärkta syster histoner. Samtidigt, kan asymmetriskt posttranslation modifierade nukleosomer genereras efter att ha använt en premodified histon kopia. Dessa preparat kan lätt användas för att studera modifieringen hörningsmekanismer, samtidigt på båda syster histoner, genom användning av högupplösande NMR-spektroskopi.

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Biokemi histoner post-translationella modifieringar nukleosomer NMR-spektroskopi epigenetik kromatin
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