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JoVE Journal Neuroscience
Imaging Neuroner inden Tykke Brain Sektioner Brug af Golgi-Cox Method
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Imaging Neuroner inden Tykke Brain Sektioner Brug af Golgi-Cox Method

Article DOI: 10.3791/55358-v • 10:26 min • April 18th, 2017
April 18th, 2017 •
1, 1, 1, 1, 1
1Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph

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Vi præsenterer en protokol for anvendelse Golgi-Cox farvningsmetode i tykke hjernesnit, for at visualisere neuroner med lange dendritiske træer indeholdt i prøver enkelt væv. To varianter af denne protokol er også præsenteret som involverer cresylviolet modfarvning, og frysning af uforarbejdede hjerner for langtidsopbevaring.

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Neuroscience neuron morfologi histologi Golgi-Cox-farvning cerebral cortex hippocampus dendritceller pigge pyramideformet neuron
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