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Journal / Genetics / Kontrollerbar Ion Channel Expression gennem…
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Genetics

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JoVE Journal Genetics
Kontrollerbar Ion Channel Expression gennem Inducible Transient transfektion
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Kontrollerbar Ion Channel Expression gennem Inducible Transient transfektion

Article DOI: 10.3791/55370-v • 10:00 min • February 17th, 2017
February 17th, 2017 •
*1, *1, 1
1The Institute for Drug Research (IDR), School of Pharmacy, Faculty of Medicine, The Hebrew University of Jerusalem (HUJI)
* These authors contributed equally

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Studere ionkanaler gennem et heterologt udtrykker system er blevet en central teknik i biomedicinsk forskning. I dette manuskript, præsenterer vi en tid effektiv metode til at opnå tæt kontrolleret ionkanal-ekspression ved at udføre transient transfektion under kontrol af en inducerbar promotor.

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Genetik transfektion HEK293 TET repressor enkelt kanal hel celle elektrofysiologi protein udtryk inducerbare udtryk heterolog ekspression calcium imaging
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